Back to Search Start Over

Fluorescence Nanoscopy of Single DNA Molecules by Using Stimulated Emission Depletion (STED)

Authors :
Persson, F.
Bingen, P.
Staudt, T.
Engelhardt, J.
Tegenfeldt, J. O.
Hell, Stefan W.
Source :
Angewandte Chemie. International Edition; June 2011, Vol. 50 Issue: 24 p5581-5583, 3p
Publication Year :
2011

Abstract

Clearing the haze: Stimulated emission depletion (STED) nanoscopy on single DNA strands reveals details with an up to five‐ to sixfold improved resolution over confocal microscopy (see images). Thus STED nanoscopy allows features to be distinguished down to the persistence length of DNA (ca. 50 nm) without promoting any significant additional photodamage, such as photobleaching or photonicking.

Details

Language :
English
ISSN :
14337851 and 15213773
Volume :
50
Issue :
24
Database :
Supplemental Index
Journal :
Angewandte Chemie. International Edition
Publication Type :
Periodical
Accession number :
ejs24424467
Full Text :
https://doi.org/10.1002/anie.201100371