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Gene Expression Profiling of Hairy Cell Leukemia Reveals a Phenotype Related to Memory B Cells with Altered Expression of Chemokine and Adhesion Receptors

Authors :
Basso, Katia
Liso, Arcangelo
Tiacci, Enrico
Benedetti, Roberta
Pulsoni, Alessandro
Foa, Robin
Di Raimondo, Francesco
Ambrosetti, Achille
Califano, Andrea
Klein, Ulf
Favera, Riccardo Dalla
Falini, Brunangelo
Source :
The Journal of Experimental Medicine; January 2004, Vol. 199 Issue: 1 p59-68, 10p
Publication Year :
2004

Abstract

Hairy cell leukemia (HCL) is a chronic B cell malignancy characterized by the diffuse infiltration of bone marrow and spleen by cells displaying a typical “hairy” morphology. However, the nature of the HCL phenotype and its relationship to normal B cells and to other lymphoma subtypes remains unclear. Using gene expression profiling, we show here that HCL displays a homogeneous pattern of gene expression, which is clearly distinct from that of other B cell non-Hodgkin lymphomas. Comparison with the gene expression profiles of purified normal B cell subpopulations, including germinal center (GC), pre-GC (naive), and post-GC (memory) B cells, shows that HCL cells are more related to memory cells, suggesting a derivation from this B cell population. Notably, when compared with memory cells, HCL cells displayed a remarkable conservation in proliferation, apoptosis, and DNA metabolism programs, whereas they appeared significantly altered in the expression of genes controlling cell adhesion and response to chemokines. Finally, these analyses have identified several genes that are specifically expressed in HCL and whose expression was confirmed at the protein level by immunocytochemical analysis of primary HCL cases. These results have biological implications relevant to the pathogenesis of this malignancy as well as clinical implications for its diagnosis and therapy.

Details

Language :
English
ISSN :
00221007 and 15409538
Volume :
199
Issue :
1
Database :
Supplemental Index
Journal :
The Journal of Experimental Medicine
Publication Type :
Periodical
Accession number :
ejs23877519
Full Text :
https://doi.org/10.1084/jem.20031175