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Bb2Bb3Regulation of Murine Lyme Arthritis Is Distinct from Ncf1and Independent of the Phagocyte Nicotinamide Adenine Dinucleotide Phosphate Oxidase

Authors :
Crandall, Hillary
Ma, Ying
Dunn, Diane M.
Sundsbak, Rhianna S.
Zachary, James F.
Olofsson, Peter
Holmdahl, Rikard
Weis, John H.
Weiss, Robert B.
Teuscher, Cory
Weis, Janis J.
Source :
American Journal of Pathology; September 2005, Vol. 167 Issue: 3 p775-785, 11p
Publication Year :
2005

Abstract

Several quantitative trait loci regulating murine Lyme arthritis severity have been mapped, including a highly significant linkage found on chromosome 5, termed Bb2Bb3. Within this region, the Ncf1gene of the phagocyte nicotinamide adenine dinucleotide phosphate (NADPH) oxidase has recently been identified as a major regulator of arthritis severity in rodent models of rheumatoid arthritis, an effect attributed to protective properties of reactive oxygen species. To assess the role of Ncf1in Lyme arthritis, we introgressed Bb2Bb3from severely arthritic C3H/He mice onto mildly arthritic C57BL/6 mice. This increased Lyme arthritis severity, whereas the reciprocal transfer conferred protection from disease. A single nucleotide polymorphism was identified in the Ncf1gene that did not influence the protein sequence or expression of Ncf1. Although polymorphonuclear leukocytes from C57BL/6 mice generated a greater oxidative burst than polymorphonuclear leukocytes from C3H/He mice, studies with the Bb2Bb3congenic mice demonstrated this difference was not linked to Ncf1alleles. Furthermore, Lyme arthritis severity was not altered in mice lacking either the Ncf1or Gp91phoxsubunits of the NADPH oxidase complex. Together, these results argue that Ncf1is not a candidate gene for regulation of Lyme arthritis and reveal Lyme arthritis to be independent of NADPH oxidase activity, distinguishing it from other models of rheumatoid arthritis.

Details

Language :
English
ISSN :
00029440
Volume :
167
Issue :
3
Database :
Supplemental Index
Journal :
American Journal of Pathology
Publication Type :
Periodical
Accession number :
ejs23571034
Full Text :
https://doi.org/10.1016/S0002-9440(10)62050-0