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Molecular and Clinical Differences between Adenocarcinomas of the Esophagus and of the Gastric Cardia

Authors :
Tanière, Philippe
Martel-Planche, Ghislaine
Maurici, Daniela
Lombard-Bohas, Catherine
Scoazec, Jean-Yves
Montesano, Ruggero
Berger, Françoise
Hainaut, Pierre
Source :
American Journal of Pathology; January 2001, Vol. 158 Issue: 1 p33-40, 8p
Publication Year :
2001

Abstract

Adenocarcinoma of the esophagus (ADCE) with Barrett’s mucosa and adenocarcinoma of the cardia (ADCC) are often reported as a single pathological entity. In this study we have used strict anatomical-pathological criteria to distinguish between these two lesions and we have investigated their differences in TP53mutations, MDM2gene amplification, and cytokeratin expression. DNA was extracted from the tumor areas of formalin-fixed, paraffin-embedded sections in 26 ADCC and 28 ADCE patients. TP53mutations were detected by temporal temperature gradient electrophoresis and identified by sequencing. MDM2 amplification was assessed by differential polymerase chain reaction. The expression of cytokeratins 4, 7, and 13 was examined by immunohistochemistry. In ADCC, the male to female ratio was 1.8:1, compared to 27:1 in ADCE. Five ADCC patients had a history of other neoplasms, compared to only one ADCE patient. The two types of tumor differed in the prevalence of TP53 mutations (31% in ADCC and 50% in ADCE) and of MDM2 gene amplification (19% in ADCC and 4% in ADCE), and in the pattern of expression of cytokeratin 7 (positive in 100% of ADCE and in 41% of ADCC) and cytokeratin 13 (positive in 81% of ADCE and in 36.5% of ADCC). ADCE and ADCC differ in their clinical characteristics, in the prevalence of TP53 mutations and MDM2 amplifications, and in the patterns of cytokeratin expression. These results support the notion that ADCC and ADCE are distinct pathological entities.

Details

Language :
English
ISSN :
00029440
Volume :
158
Issue :
1
Database :
Supplemental Index
Journal :
American Journal of Pathology
Publication Type :
Periodical
Accession number :
ejs23566177
Full Text :
https://doi.org/10.1016/S0002-9440(10)63941-7