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Detection of Enterobacterial Lipopolysaccharides and Experimental Endotoxemia by Means of an Immunolimulus Assay Using Both SerotypeSpecific and Cross-Reactive Antibodies
- Source :
- The Journal of Infectious Diseases; August 1993, Vol. 168 Issue: 2 p393-393, 1p
- Publication Year :
- 1993
-
Abstract
- The immunolimulus (IML) assay system uses solid-phase endotoxin antibodies to capture lipopolysaccharide (LPS), which is then quantified by a modification of the chromogenic limulus amebocyte lysate (CLAL) method. Monoclonal antibodies (MAbs) reactive with selected 0 antigen serotypes of Escherichia coli</it> (O18) and Salmonella typhimurium (O-9,12), when used in the IML, were shown to be highly specific in detecting their respective endotoxins in purified form and in plasma samples from experimentally infected animals. A murine MAb that was broadly cross-reactive with E. coli, Salmonella,</it> and Shigella</it> endotoxins also proved to be highly effective in the IML assay for capturing LPS molecules from both E. coli</it> and S. typhimurium</it> strains. These results indicate that IML assays can detect smooth-type enterobacterial endotoxins in plasma and suggest that such assays have potential for use in the rapid diagnosis of sepsis and endotoxemia caused by different enterobacterial species.
Details
- Language :
- English
- ISSN :
- 00221899 and 15376613
- Volume :
- 168
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- The Journal of Infectious Diseases
- Publication Type :
- Periodical
- Accession number :
- ejs23127905
- Full Text :
- https://doi.org/10.1093/infdis/168.2.393