Back to Search Start Over

A fast screening method for the identification of siderophores from fluorescent <TOGGLE>Pseudomonas</TOGGLE> spp. by liquid chromatography/electrospray mass spectrometry<FN ID="fn1">Part LXXIX of the series ‘‘Bacterial Constituents;’’ for part LXXVIII see Ref. 16. This paper is dedicated to the memory of Prof. Dr Wilhelm Richter</FN>

Authors :
Kilz, S.
Lenz, Ch.
Fuchs, R.
Budzikiewicz, H.
Source :
Journal of Mass Spectrometry; April 1999, Vol. 34 Issue: 4 p281-290, 10p
Publication Year :
1999

Abstract

A screening method was developed for the fast identification of known pyoverdin-type siderophores produced by fluorescent Pseudomonas spp. It is based on reversed-phase high-performance liquid chromatography interfaced with electrospray ionization mass spectrometry of the Sep-Pak RP-C&lt;INF&gt;18&lt;/INF&gt; culture supernatant extracts. The siderophores of five bacterial strains were characterized by their molecular masses obtained from their doubly protonated molecular ions [M+2H]&lt;SUP&gt;2+&lt;/SUP&gt; and their UV/visible spectra recorded with a diode-array detector. Additional structural information was gained by skimmer collision-induced dissociation experiments. For all strains new minor siderophores were found. A table of fully or partially identified pyoverdins and related siderophores is provided which will be the basis for screening studies. Copyright &#169; 1999 by John Wiley &amp; Sons, Ltd.

Details

Language :
English
ISSN :
10765174 and 10969888
Volume :
34
Issue :
4
Database :
Supplemental Index
Journal :
Journal of Mass Spectrometry
Publication Type :
Periodical
Accession number :
ejs2201835
Full Text :
https://doi.org/10.1002/(SICI)1096-9888(199904)34:4<281::AID-JMS750>3.0.CO;2-M