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A fast screening method for the identification of siderophores from fluorescent <TOGGLE>Pseudomonas</TOGGLE> spp. by liquid chromatography/electrospray mass spectrometry<FN ID="fn1">Part LXXIX of the series Bacterial Constituents; for part LXXVIII see Ref. 16. This paper is dedicated to the memory of Prof. Dr Wilhelm Richter</FN>
- Source :
- Journal of Mass Spectrometry; April 1999, Vol. 34 Issue: 4 p281-290, 10p
- Publication Year :
- 1999
-
Abstract
- A screening method was developed for the fast identification of known pyoverdin-type siderophores produced by fluorescent Pseudomonas spp. It is based on reversed-phase high-performance liquid chromatography interfaced with electrospray ionization mass spectrometry of the Sep-Pak RP-C<INF>18</INF> culture supernatant extracts. The siderophores of five bacterial strains were characterized by their molecular masses obtained from their doubly protonated molecular ions [M+2H]<SUP>2+</SUP> and their UV/visible spectra recorded with a diode-array detector. Additional structural information was gained by skimmer collision-induced dissociation experiments. For all strains new minor siderophores were found. A table of fully or partially identified pyoverdins and related siderophores is provided which will be the basis for screening studies. Copyright © 1999 by John Wiley & Sons, Ltd.
Details
- Language :
- English
- ISSN :
- 10765174 and 10969888
- Volume :
- 34
- Issue :
- 4
- Database :
- Supplemental Index
- Journal :
- Journal of Mass Spectrometry
- Publication Type :
- Periodical
- Accession number :
- ejs2201835
- Full Text :
- https://doi.org/10.1002/(SICI)1096-9888(199904)34:4<281::AID-JMS750>3.0.CO;2-M