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Proteomic and Functional Characterization of a Chlamydomonas reinhardtiiMutant Lacking the Mitochondrial Alternative Oxidase 1
- Source :
- Journal of Proteome Research; 20240101, Issue: Preprints
- Publication Year :
- 2024
-
Abstract
- In the present work, we have isolated by RNA interference and characterized at the functional and the proteomic levels a Chlamydomonas reinhardtiistrain devoid of the mitochondrial alternative oxidase 1 (AOX1). The AOX1-deficient strain displays a remarkable doubling of the cell volume and biomass without alteration of the generation time or change in total respiratory rate, with a significantly higher ROS production. To identify the molecular adaptation underlying these observations, we have carried out a comparative study of both the mitochondrial and the cellular soluble proteomes. Our results indicate a strong up-regulation of the ROS scavenging systems and important quantitative modifications of proteins involved in the primary metabolism, namely an increase of enzymes involved in anabolic pathways and a concomitant general down-regulation of enzymes of the main catabolic pathways.
Details
- Language :
- English
- ISSN :
- 15353893 and 15353907
- Issue :
- Preprints
- Database :
- Supplemental Index
- Journal :
- Journal of Proteome Research
- Publication Type :
- Periodical
- Accession number :
- ejs21200999
- Full Text :
- https://doi.org/10.1021/pr900866e