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Arylacetamide deacetylase attenuates fatty-acid-induced triacylglycerol accumulation in rat hepatoma cells
- Source :
- Journal of Lipid Research; February 2010, Vol. 51 Issue: 2 p368-377, 10p
- Publication Year :
- 2010
-
Abstract
- Mobilization of hepatic triacylglycerol stores provides substrates for mitochondrial β-oxidation and assembly of VLDLs; however, the identity of lipolytic enzymes involved in the regulation of this process remains largely unknown. Arylacetamide deacetylase (AADA) shares homology with hormone-sensitive lipase and therefore could potentially participate in hepatic lipid metabolism, including the regulation of hepatic triacylglycerol levels. We have established McArdle-RH7777 (rat hepatoma) cell lines stably expressing mouse AADA cDNA and performed metabolic labeling as well as lipid mass analyses. Expression of AADA cDNA in McArdle-RH7777 cells significantly reduced intracellular triacylglycerol levels and apolipoprotein B secretion and increased fatty acid oxidation.
Details
- Language :
- English
- ISSN :
- 00222275 and 15397262
- Volume :
- 51
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Journal of Lipid Research
- Publication Type :
- Periodical
- Accession number :
- ejs20542247