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Phosphoproteomics for the Masses
- Source :
- ACS Chemical Biology; January 2010, Vol. 5 Issue: 1 p105-119, 15p
- Publication Year :
- 2010
-
Abstract
- Protein phosphorylation serves as a primary mechanism of signal transduction in the cells of biological organisms. Technical advancements over the last several years in mass spectrometry now allow for the large-scale identification and quantitation of in vivophosphorylation at unprecedented levels. These developments have occurred in the areas of sample preparation, instrumentation, quantitative methodology, and informatics so that today, 10 000−20 000 phosphorylation sites can be identified and quantified within a few weeks. With the rapid development and widespread availability of such data, its translation into biological insight and knowledge is a current obstacle. Here we present an overview of how this technology came to be and is currently applied, as well as future challenges for the field.
Details
- Language :
- English
- ISSN :
- 15548929 and 15548937
- Volume :
- 5
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- ACS Chemical Biology
- Publication Type :
- Periodical
- Accession number :
- ejs20494179
- Full Text :
- https://doi.org/10.1021/cb900277e