Back to Search
Start Over
RNA-binding protein conserved in both microtubule- and microfilament-based RNA localization.
- Source :
- Genes & Development; June 1998, Vol. 12 Issue: 11 p1593-1598, 6p
- Publication Year :
- 1998
-
Abstract
- Vg1 mRNA translocation to the vegetal cortex of Xenopus oocytes requires intact microtubules, and a 3' UTR cis-acting element (termed VLE), which also mediates sequence-specific binding of several proteins. One protein, the 69-kD Vg1 RBP, associates Vg1 RNA to microtubules in vitro. Here we show that Vg1 RBP-binding sites correlate with vegetal localization. Purification and cloning of Vg1 RBP revealed five RNA-binding motifs: four KH and one RRM domains. Surprisingly, Vg1 RBP is highly homologous to the zipcode binding protein implicated in the microfilament-mediated localization of beta actin mRNA in fibroblasts. These data support Vg1 RBP's direct role in vegetal localization and suggest the existence of a general, evolutionarily conserved mechanism for mRNA targeting.
Details
- Language :
- English
- ISSN :
- 08909369 and 15495477
- Volume :
- 12
- Issue :
- 11
- Database :
- Supplemental Index
- Journal :
- Genes & Development
- Publication Type :
- Periodical
- Accession number :
- ejs18910185