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The tetracycline resistance determinants of RP1 and Tnl721: nucleotide sequence analysis
- Source :
- Nucleic Acids Research; September 1983, Vol. 11 Issue: 17 p6089-6089, 1p
- Publication Year :
- 1983
-
Abstract
- Nucleotide sequences of the homologous tetracycline resistance (tet) determinants of plasmid RP1 and transposon Tn1721 have been determined. Two open reading frames of divergent polarity have been assigned to a regulatory gene (tetR) and a gene encoding a resistance protein (tetA). The intercistronic region contains appropriate regulatory and transcription signals. The tetR gene can code for a protein of 216 araino acids (deduced mol.wt. 23,288) and the tetA gene for a protein of 399 amino acids (deduced raol. wt. 42,205). Based on the deduced amino acid sequence, the tetA proteins of RP1/Tn1721 are 78% homologous with that of pBR322 and 45% homologous with that of TnlO. We conclude that a single tetA gene mediates resistance in each of these tet determinants.
Details
- Language :
- English
- ISSN :
- 03051048 and 13624962
- Volume :
- 11
- Issue :
- 17
- Database :
- Supplemental Index
- Journal :
- Nucleic Acids Research
- Publication Type :
- Periodical
- Accession number :
- ejs18584451
- Full Text :
- https://doi.org/10.1093/nar/11.17.6089