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Expression and immunogenicity in rats of recombinant adenovirus 5 DNA plasmids and vaccinia virus containing the HTLV-I-env gene
- Source :
- International Journal of Cancer; 10 April 1997, Vol. 71 Issue: 2 p300-307, 8p
- Publication Year :
- 1997
-
Abstract
- The complete human T-cell leukemia virus type I (HTLV-I) env gene was inserted into an expression cassette containing the adenovirus 5 major late promoter (Ad5-MLP). Recombinant Ad5-HTLV-I-env was obtained by homologous recombination in 293 cells simultaneously transfected by the expression cassette and the genomic DNA of Ad5. In vitro expression of the HTLV-I-env gene in the recombinant vector was detected by immunofluorescence and Western blotting. Functional expression of HTLV-I-env was confirmed by syncitium formation specifically in HeLa cells infected with Ad5-HTLV-I-env. Two immunization regimens against HTLV-I were tested in WKY and Fischer F-344 rats. The first involved WKY rats primed with Ad5-HTLV-I-env or naked DNA plasmids containing the HTLV-I-env gene and boosted with Ad5 containing the HTLV-I-env gp46 gene or with baculovirusderived recombinant gp46. No antibody against HTLV-I was detected, while HTLV-I-specific cytotoxic T lymphocytes were recovered from all immunized groups but not from controls. The second approach involved Fischer F-344 rats primed and boosted with recombinant vaccinia virus containing the HTLV-I-env gene. Such rats developed antibodies against the HTLV-I env gp21 and gp46 (non-neutralizing). After challenge with human HTLV-I-producing cells (MT-2), both immunization regimens were found to induce partial protection. Int. J. Cancer 71:300307, 1997. © 1997 Wiley-Liss, Inc.
Details
- Language :
- English
- ISSN :
- 00207136 and 10970215
- Volume :
- 71
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- International Journal of Cancer
- Publication Type :
- Periodical
- Accession number :
- ejs1846991
- Full Text :
- https://doi.org/10.1002/(SICI)1097-0215(19970410)71:2<300::AID-IJC27>3.0.CO;2-J