Immunophenotypic profile of acute leukemia: Critical analysis and insights gained at a tertiary care center in IndiaHow to cite this article: Gujral S, Badrinath Y, Kumar A, Subramanian PG, Raje G, Jain H, Pais A, Kadam PSA, Banavali SD, Arora B, Kumar P, Hari Menon VG, Kurkure PA, Parikh PM, Mahadik S, Chogule AB, Shinde SC, Nair CN. Immunophenotypic profile of acute leukemia: critical analysis and insights gained at a tertiary care center in India. Cytometry Part B 2009; 76B: 199–205.

BackgroundTo analyze the spectrum of various types and subtypes of acute leukemia.MethodsTwo thousand five hundred and eleven consecutive new referral cases of acute leukemia AL were evaluated based on WHO classification.ResultsIt included 1,471 cases 58 of acute lymphoblastic leukemia ALL, 964 cases 38 of acute myeloid leukemia AML, 45 cases 1.8 of chronic myelogenous leukemia in blast crisis CMLBC, 37 cases 1.5 of biphenotypic acute leukemia BAL, 1 case of Triphenotypic AL, and 2 cases of acute undifferentiated leukemia AUL. Common subtypes of ALL were Bcell ALL 76, which comprised of intermediate stageCALLA positive 73, early precursorproBALL 3. Tcell ALL constituted 24 351 cases of ALL. Common subtypes of AML included AMLM2 27, AMLM5 15, AMLM0 12, AMLM1 12, APML 11, and AML t8;21 9. CMLBC was commonly of myeloid blast crisis subtype 40 cases.ConclusionBcell ALL was the commonest subtype in children and AML in adults. Overall incidence of AML in adults was low 53 only. CD13 was most sensitive and CD117 most specific for determining myeloid lineage. A minimal primary panel of nine antibodies consisting of three myeloid markers CD13, CD33, and CD117, Bcell lymphoid marker CD19, Tcell marker CD7, with CD45, CD10, CD34, and HLADR could assign lineage to 92 of AL. Cytogenetics findings lead to a change in the diagnostic subtype of myeloid malignancy in 38 1.5 cases. © 2008 Clinical Cytometry Society