Direct enantiomeric HPLC separation of several 2-arylpropionic acids, barbituric acids and benzodiazepines on chiralcel OJ-R chiral stationary phase

Summary: A newly developed reversed-phase chiral stationary phase, cellulose tris(4-methylbenzoate), known as Chiralcel OJ-R, has been successfully applied to the direct resolution of the enantiomers of several nonsteroidal anti-inflammatory drugs belonging to the profen group. Among the five barbituric acids tested, the chiral resolution of mephobarbital is especially high. The Chiralcel OJ-R column also proved quite efficient for the enantiomeric resolution of a few representatives of the benzodiazepine group.Variations of mobile phase conditions based on acetonitrile and/or methanol were tested. The results obtained are briefly compared with separations performed using a Chiralcel OJ column under normalphase conditions.