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Signal Perception and Intracellular Signal Transduction in Plant Pathogen Defense
- Source :
- Journal of Receptor and Signal Transduction Research; January 1997, Vol. 17 Issue: 1-3 p127-136, 10p
- Publication Year :
- 1997
-
Abstract
- AbstractDisease resistance in plant/pathogen interactions requires sensitive and specific recognition mechanisms for pathogen-derived signals in plants. Cultured parsley (Petroselinum crispum) cells respond to treatment with a crude cell wall preparation derived from the phytopathogenic fungus Phytophthora sojaewith transcriptional activation of the same set of defense-related genes as are activated in parsley leaves upon infection with fungal spores. A 13 amino acid core sequence (Pep-13) of a 42 kDa fungal cell wall glycoprotein was identified, which stimulates the same responses as the crude cell wall elicitor, namely macroscopic Ca2and H-influxes, effluxes of K- and Cl−ions, production of active oxygen species (oxidative burst), defense-related gene activation, and formation of antifungal phytoalexins. Using [125I]Tyr-Pep-13 as ligand in binding assays, a single-class high-affinity binding site in parsley microsomal membranes and protoplasts could be detected. Binding was specific, saturable, and reversible. By chemical crosslinking, a 91 kDa parsley plasma membrane protein was identified to be the receptor of the peptide elicitor. Isolation of this receptor protein involved in pathogen defense in plants is under way.
Details
- Language :
- English
- ISSN :
- 10799893 and 15324281
- Volume :
- 17
- Issue :
- 1-3
- Database :
- Supplemental Index
- Journal :
- Journal of Receptor and Signal Transduction Research
- Publication Type :
- Periodical
- Accession number :
- ejs17079491
- Full Text :
- https://doi.org/10.3109/10799899709036598