Back to Search
Start Over
Protein Preparation, Crystallization and Preliminary X-Ray Crystallographic Analysis of N-Acetylglutamate Kinase from Streptococcus mutans
- Source :
- Protein and Peptide Letters; June 2008, Vol. 15 Issue: 5 p541-543, 3p
- Publication Year :
- 2008
-
Abstract
- The N-acetylglutamate kinase from Streptococcus mutans was expressed in Escherichia coli in soluble form and purified to homogeneity. Crystals suitable for X-ray diffraction were obtained by hanging-drop vapor diffusion method and diffracted to 2.06 Å. The crystal belonged to space group P21212, with unit cell parameters a 57.19 Å, b 94.76 Å, c 47.58 Å. The gel filtration and initial phasing results showed that the enzyme exists as a monomer, which is different from previously reported N-acetylglutamate kinases.
Details
- Language :
- English
- ISSN :
- 09298665
- Volume :
- 15
- Issue :
- 5
- Database :
- Supplemental Index
- Journal :
- Protein and Peptide Letters
- Publication Type :
- Periodical
- Accession number :
- ejs16891664