Neutronenaktivierungsanalytische Bestimmung des Chromgehaltes von Laboratoriumskaninchen-Skelettmuskel

The accurate values of the chromium contents of human and mammalian tissues and body fluids and appropriate procedures for their determination are still a matter of discussion. In this connexion, the chromium content of skeletal muscle of laboratory rabbits has been determined using neutron activation analysis. Data hitherto published by other authors: <7.1–121 ng/g. The procedure for separation of ⁵¹Cr by distillation of chromium oxychloride, described in the literature, has been improved. The arrangements necessary to minimize the chromium blank values are described. The main component of this blank is caused by the residual chromium contamination of the surface of the sample vial; typical values of this component are 0.049 ng Cr (without lyophilization) and 0.12 ng Cr (with lyophilization). The analyses of standard reference materials (SRM) yielded values of the chromium contents that are in agreement (i) with the certified value in the case of NBS Citrus Leaves, and (ii) with the latest published value of 9.2±2.5 ng/g in the case of IAEA animals muscle (H-4). NBS Orchard Leaves was found not to be an appropriate SRM for testing the method. In analyses of samples of thigh muscle of bastard rabbits chromium contents of 6.2–22.9 ng/g (fresh weight basis) were obtained. Comparison of these data with a previously found value of 1.2 ng/g, the literature value <7.1 ng/g and the value 2.5 ng/g for H-4 calculated on fresh weight basis indicated that the chromium contents of mammalian skeletal muscle might lie in a broad range, even for a subspecies.