Paper electrophoresis of steroids

A radiochemical method for the identification of some steroids on electropherograms is described. A comparison between this method and another modified spectrophotometric method is given. The radiochemical method can be used for quantitative work; the relationship between the concentration of the hydrazones and the radioactivity gained by the steroids is linear. This technique is superior to any colorimetric one, it needs less reagents and is not time consuming. Application of the method to other steroids is continued.