Histamin in der Interstitialflüssigkeit der befallenen und klinisch nicht befallenen Haut bei chronisch rezidivierender Urticaria

In 8 patients with idiopathic chronic urticaria suction blisters were produced simultaneously on lesional and perilesional skin in the periumbilical area without application of heat. The suction pressure was 250 mm Hg, and thus the blister production lasted from 60–120 min. The blister fluid histamine was determined with a modified autoanalyzer technique developed by Siraganian. In this method the extraction of histamine, the condensation of o-phthaldialdehyde with histamine and the measurement of the fluorochrome is automated. With the described method it is possible to measure values less than 0.5 ng/ml.