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Absence of calcium channels in neonatal rat aortic myocytes
- Source :
- Pflügers Archiv European Journal of Physiology; March 1996, Vol. 431 Issue: 5 p791-793, 3p
- Publication Year :
- 1996
-
Abstract
- We have investigated whole-cell Ba<superscript>2+</superscript> currents through Ca<superscript>2+</superscript> channels (I<subscript>Ba</subscript>) in single myocytes freshly isolated from the aortic media of neonatal (1-day-old) and adult (12-week-old) rats. In neonatal myocytes, I<subscript>Ba</subscript> was undetectable even in presence of the dihydropyridine (DHP) agonist Bay K 8644. Binding of [<superscript>3</superscript>H]Nitrendipine on crude plasma membrane preparation of media confirmed the absence of DHP-receptors. By contrast, a robust DHP-sensitive ‘L-type’ I<subscript>Ba</subscript> was recorded in adults which was consistent with the presence of specific [<superscript>3</superscript>H]Nitrendipine binding sites. In conclusion, neonatal aortic myocytes do not express any Ca<superscript>2+</superscript> channels. The acquisition of L-type Ca<superscript>2+</superscript> channels may be related to cell differentiation and acquisition of contractility during postnatal development.
Details
- Language :
- English
- ISSN :
- 00316768 and 14322013
- Volume :
- 431
- Issue :
- 5
- Database :
- Supplemental Index
- Journal :
- Pflügers Archiv European Journal of Physiology
- Publication Type :
- Periodical
- Accession number :
- ejs16364270
- Full Text :
- https://doi.org/10.1007/BF02253845