Back to Search
Start Over
Adriamycin binding assay: a valuable chemosensitivity test in human osteosarcoma
- Source :
- Journal of Cancer Research and Clinical Oncology; February 1992, Vol. 119 Issue: 2 p121-126, 6p
- Publication Year :
- 1992
-
Abstract
- Summary The reliability of a simple method evaluating the pattern of subcellular binding of Adriamycin (Adriamycin binding assay, ABA) as an index of sensitivity was demonstrated in different primary cultures and in sensitive and resistant cell lines of human osteosarcoma. After exposure to Adriamycin (10 µg/ml for 30 min at 37°C), living sensitive cells showed selective intranuclear uptake of the drug, whereas in resistant cells no distinct subcellular distribution was observed. The binding pattern of Adriamycin in sensitive and in highly resistant cells was inversely related to the expression of P-glycoprotein. However, low levels of resistance in vitro, not detectable by increased levels of expression of P-glycoprotein, were revealed by ABA. The use of ABA in combination with the estimate of P-glycoprotein expression is recommended in clinical practice as an accurate means for predicting the sensitivity of osteosarcoma to Adriamycin.
Details
- Language :
- English
- ISSN :
- 01715216 and 14321335
- Volume :
- 119
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Journal of Cancer Research and Clinical Oncology
- Publication Type :
- Periodical
- Accession number :
- ejs16097551
- Full Text :
- https://doi.org/10.1007/BF01209668