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Fermentation strategies for recombinant protein expression in the methylotrophic yeastPichia pastoris
- Source :
- Biotechnology and Bioprocess Engineering; August 2000, Vol. 5 Issue: 4 p275-287, 13p
- Publication Year :
- 2000
-
Abstract
- Fermentation strategies for recombinant protein production inPichia pastorishave been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, application of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail.
Details
- Language :
- English
- ISSN :
- 12268372 and 19763816
- Volume :
- 5
- Issue :
- 4
- Database :
- Supplemental Index
- Journal :
- Biotechnology and Bioprocess Engineering
- Publication Type :
- Periodical
- Accession number :
- ejs15880231
- Full Text :
- https://doi.org/10.1007/BF02942184