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Effects of leflunomide (HWA 486) on expression of lymphocyte activation markers

Authors :
Zielinski, Th.
Müller, H. -J.
Bartlett, R. R.
Source :
Inflammation Research; June 1993, Vol. 38 Issue: Supplement 2 pC80-C82, 3p
Publication Year :
1993

Abstract

Leflunomide (HWA 486), an isoxazol derivative, has been shown to be very effective in combating autoimmune diseases and transplantation rejection in a great number of animal models. The main metabolite of leflunomide, A77 1726, is a potent antiproliferative compound. To further elucidate this affect, lymphocytes of healthy human donors were cultured for 24, 48 or 72h in the presence of PHA or immobilized anti-CD3 antibody. A77 1726 was added at concentrations between 10 and 100 μM. Flow cytometric evaluation of early activation or proliferation markers (IL-2 and transferrin receptors, respectively) showed that their expression was inhibited in a dose-dependent manner by A77 1726. Together with previous data, these experiments indicate that leflunomide not only inhibits the expansion of already proliferating lymphocytes, but also impairs PHA and anti-CD3 antibody triggered activation of quiescent cells. Thus, this compound may exert its effects through influencing two important aspects of an immune response, that is, activation and proliferation of lymphocytes.

Details

Language :
English
ISSN :
10233830 and 1420908X
Volume :
38
Issue :
Supplement 2
Database :
Supplemental Index
Journal :
Inflammation Research
Publication Type :
Periodical
Accession number :
ejs15702458
Full Text :
https://doi.org/10.1007/BF01991144