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Current triton X-100 treatments do not allow a complete plasminogen activator extraction from developing nervous tissue
- Source :
- Neurochemical Research; February 1995, Vol. 20 Issue: 2 p137-142, 6p
- Publication Year :
- 1995
-
Abstract
- Determinations of plaminogen activator (PA) activity are usually performed in Triton X-100-treated tissue homogenates or crude membrane fractions. Such preparations usually involve a single Triton X-100 treatment. In the present paper we describe the pattern of variability of PA activity measured in different fractions obtained from the developing chick CNS by a repetitive procedure of Triton X-100 treatment and ultracentrifugation. To further characterize this PA activity we have also performed zymographic analyses during the embryonic development and the early postnatal life. Our results show that: a) a single Triton X-100 treatment does not completely extract the enzyme and this lead to an underestimation of the total PA activity; b) the PA activity is associated with the particulate component of the total tissue homogenate requiring its complete solubilization more drastic Triton X-100 treatments; c) better estimations of total and specific activities are obtained by using soluble fractions derived by ultracentrifugation from Triton X-100-treated membrane fractions; d) the developing chick optic lobe expresses only one kind of PA molecule along the entire development; e) the level of PA activity vary characteristically during the ontogeny and the early postnatal life indicating the existence of a developmentally regulated mechanism of PA expression.
Details
- Language :
- English
- ISSN :
- 03643190 and 15736903
- Volume :
- 20
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Neurochemical Research
- Publication Type :
- Periodical
- Accession number :
- ejs15445029
- Full Text :
- https://doi.org/10.1007/BF00970537