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Monoclonal antibodies to rabbit liver cathepsin B
- Source :
- Bioscience Reports; July 1986, Vol. 6 Issue: 7 p639-646, 8p
- Publication Year :
- 1986
-
Abstract
- Three stable hybridoma cell lines (AF8, BC11, CE2) have been produced that secrete antibodies specific for cathepsin B. These have been characterized by ELISA, SDS-PAGE immunostaining, immunoprecipitation and immunofluorescent staining. CE2 immunoprecipitated native cathepsin B with retention of enzymic activity, but failed to cross-react with the alkali-denatured enzyme. BC11 bound only to the denatured form of cathepsin B and AF8 cross-reacted with both native and denatured cathepsin B. However, unlike CE2-immunoprecipitated enzyme, activity could be detected only after dissociation of the antigen-AF8 antibody complex. No cross reaction was found with any lysosomal protein includihg the cysteine proteinases, catbepsins H and L.
Details
- Language :
- English
- ISSN :
- 01448463 and 15734935
- Volume :
- 6
- Issue :
- 7
- Database :
- Supplemental Index
- Journal :
- Bioscience Reports
- Publication Type :
- Periodical
- Accession number :
- ejs15091849
- Full Text :
- https://doi.org/10.1007/BF01114758