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Ginkgo Biloba Extract EGB 761 or Trolox C Prevent the Ascorbic Acid/FE2+ Induced Decrease in Synaptosomal Membrane Fluidity
- Source :
- Free Radical Research; 1993, Vol. 19 Issue: 5 p341-350, 10p
- Publication Year :
- 1993
-
Abstract
- The ability of synaptosomes, prepared from striata, to take up 3H-dopamine declined rapidly during incubation at 37°C, in an oxygenated Krebs-Ringer medium with 0.1 mM ascorbic acid. Ascorbic acid was responsible for this decrease. Its effectiveness after a 60 min incubation was concentration dependent from 1 μM and virtually complete for 0.1 mM. Furthermore, a decrease of synaptosomal membrane fluidity was revealed by measurements of fluorescence polarization using 1,6-diphenyl-1,3,5-hexatriene. This decrease was potentiated by Fe2+ ions (1 μM). In contrast, it was prevented by the Fe2+ ion chelator, desferrioxamine (0.1 mM), by the Ginkgo biloba extract EGb 761 [2-16 μg/ml], as well as by the flavonoid quercetin (0.1 μM). This preventive effect was shared by trolox C (from 0.1 mM). It is concluded that peroxidation of neuronal membrane lipids induced by ascorbic acid/Fe2+ is associated with a decrease in membrane fluidity which, in turn, reduces the ability of the dopamine transporter to take up dopamine.
Details
- Language :
- English
- ISSN :
- 10715762 and 10292470
- Volume :
- 19
- Issue :
- 5
- Database :
- Supplemental Index
- Journal :
- Free Radical Research
- Publication Type :
- Periodical
- Accession number :
- ejs13108001
- Full Text :
- https://doi.org/10.3109/10715769309056523