Selection of Columns for Analysis of Blood Urea

For the analysis of blood urea, the author used several kinds of columns such as conventional ion exchange chromatographic column, ion ehromatographic column and reverse phase columns. The result of comparison for separation efficiency will be discussed and conventional ion exchange chromatographic column indicated a best separation efficiency. Prior to application to the columns, blood urea was treated with solid phase extraction (SPE) with a strong cation exchange column with SO3H functional group.