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A Highly Sensitive Enzyme Immunoassay of Anti-Insulin Antibodies in Guinea Pig Serum
- Source :
- Analytical Letters; January 1986, Vol. 19 p1083-1095, 13p
- Publication Year :
- 1986
-
Abstract
- A highly sensitive enzyme immunoassay of anti-insulin antibodies in guinea pig serum is described. Guinea pig anti-insulin serum was diluted to various extents with nonspecific guinea pig serum and incubated with insulin. Insulin bound to anti-insulin antibodies was separated from free insulin by precipitation with polyethylene glycol. Anti-insulin antibodies in the precipitates were dissociated from insulin and inactivated by incubation with 0.1 mol/1 HCl. The amount of insulin dissociated was measured by sandwich enzyme immunoassay using anti-insulin IgG-coated polystyrene balls and affinity-purified anti-insulin Fab′-horseradish peroxidase conjugate. The detection limit of anti-insulin antibodies in guinea pig serum was improved 1,000-fold as compared with that of the enzyme immunoassay previously described, in which insulin-coated polystyrene balls were incubated with diluted guinea pig anti-insulin serum and subsequently with rabbit (anti-guinea pig IgG) Fab′-horseradish peroxidase conjugate.
Details
- Language :
- English
- ISSN :
- 00032719 and 1532236X
- Volume :
- 19
- Database :
- Supplemental Index
- Journal :
- Analytical Letters
- Publication Type :
- Periodical
- Accession number :
- ejs11086351
- Full Text :
- https://doi.org/10.1080/00032718608066284