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Use of cyanobacterial gas vesicles as oxygen carriers in cell culture

Use of cyanobacterial gas vesicles as oxygen carriers in cell culture

Authors :
Sundararajan, Anand
Ju, Lu-Kwang
Source :
Cytotechnology; October 2006, Vol. 52 Issue: 2 p139-149, 11p
Publication Year :
2006

Abstract

Abstract: The gas vesicles isolated from the cells of filamentous cyanobacterium Anabaena flos-aquae were treated and sterilized with glutaraldehyde and then evaluated for their effectiveness as gas carriers in cell culture. Anchorage-dependent Vero cells were grown in a packed bed of microcarrier beads under the perfusion of Dulbecco’s Modified Eagle’s Medium with 1% serum. The culture medium supplemented with 1.8% (v/v) gas vesicles was found to support a 30% higher maximum glucose utilization rate than the same medium without gas vesicles. The gas vesicle suspension was confirmed to have no apparent effects on cell metabolism in T-flask cultures. The study results indicated that the gas vesicles, with high oxygen carrying capacity, can be used to increase the oxygen supply in cell culture systems.

Details

Language :
English
ISSN :
09209069 and 15730778
Volume :
52
Issue :
2
Database :
Supplemental Index
Journal :
Cytotechnology
Publication Type :
Periodical
Accession number :
ejs11073620
Full Text :
https://doi.org/10.1007/s10616-007-9044-9