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Use of cyanobacterial gas vesicles as oxygen carriers in cell culture
Use of cyanobacterial gas vesicles as oxygen carriers in cell culture
- Source :
- Cytotechnology; October 2006, Vol. 52 Issue: 2 p139-149, 11p
- Publication Year :
- 2006
-
Abstract
- Abstract: The gas vesicles isolated from the cells of filamentous cyanobacterium Anabaena flos-aquae were treated and sterilized with glutaraldehyde and then evaluated for their effectiveness as gas carriers in cell culture. Anchorage-dependent Vero cells were grown in a packed bed of microcarrier beads under the perfusion of Dulbecco’s Modified Eagle’s Medium with 1% serum. The culture medium supplemented with 1.8% (v/v) gas vesicles was found to support a 30% higher maximum glucose utilization rate than the same medium without gas vesicles. The gas vesicle suspension was confirmed to have no apparent effects on cell metabolism in T-flask cultures. The study results indicated that the gas vesicles, with high oxygen carrying capacity, can be used to increase the oxygen supply in cell culture systems.
Details
- Language :
- English
- ISSN :
- 09209069 and 15730778
- Volume :
- 52
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Cytotechnology
- Publication Type :
- Periodical
- Accession number :
- ejs11073620
- Full Text :
- https://doi.org/10.1007/s10616-007-9044-9