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Molecular Cloning and Characterization of the Expression Profiles of Vitellogenin Transcripts in the Dojo Loach ( Misgurnus anguillicaudatus) in Response to 17α-Ethinylestradiol and 17β-Estradiol Administration.
- Source :
- Zoological Science (Zoological Society of Japan); Apr2014, Vol. 31 Issue 4, p202-2012, 11p
- Publication Year :
- 2014
-
Abstract
- The gene, vitellogenin ( vtg) was cloned and characterized in the dojo loach ( Misgurnus anguillicaudatus), an indigenous freshwater species in East Asia, in order to develop tools for detecting the effects of estrogenic endocrine-disrupting chemicals (EEDCs). Full-length cDNAs encoding seven distinct vtg transcripts ( vtg1-7) were obtained. The corresponding deduced amino acid sequences (Vtg1-7) were divided into two types; type I (Vtg1-6; 89-99% identical), which contained both lipovitellin (Lv) and phosvitin (Pv), and type II (Vtg7), which contained Lv alone. Phylogenetic analysis revealed that the type I and type II Vtgs in the loach could be classified as VtgAo1 and VtgC types, respectively. Immuno-biochemical analyses using type-specific Vtg antisera revealed that VtgAo1 proteins appeared to be the major Vtg type in this species. Males were administered (aqueous exposure) either 17β-estradiol (E2) or 17α-ethinylestradiol (EE2), the results from which were used to determine that hepatic vtgAo1 expression was estrogen-sensitive. The precise classification of the loach vtg/Vtg products, as well as their induction profiles following the estrogenic stimulation, provide a basis for their use as sensitive biomarkers when EEDC activities are evaluated in the freshwater environments in East Asia. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02890003
- Volume :
- 31
- Issue :
- 4
- Database :
- Supplemental Index
- Journal :
- Zoological Science (Zoological Society of Japan)
- Publication Type :
- Periodical
- Accession number :
- 95342095
- Full Text :
- https://doi.org/10.2108/zs130223