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The study on expression and activity of New Delhi metal-ß-lactamase-1.

Authors :
XIA Li-liang
ZHANG Rui-an
LIU Jun
WANG Zhong-qiang
ZHU Ling-wei
JI Xue
LIU Nan
CHEN Shuo
SONG Hong-bin
SUN Yang
FENG Shu-zhang
Source :
Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao; oct2013, Vol. 33 Issue 10, p1573-1578, 6p
Publication Year :
2013

Abstract

The article discusses a study on expression and activity of New Delhi metal-ß-lactamase-1 based on culture temperature, inducing time and Isopropyl ß-D-1-thiogalactopyranoside concentration to study expression conditions of recombinant protein. It states that protein was identified by matrix-assisted laser desorption-ionization (MALDI-TOF-MS) and ethylenediaminetetraacetic acid (EDTA) has an inhibitory effect on fusion protein enzymatic activity in vitro.

Details

Language :
Chinese
ISSN :
10054545
Volume :
33
Issue :
10
Database :
Supplemental Index
Journal :
Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao
Publication Type :
Academic Journal
Accession number :
93454380