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Antigen-loaded nanocarriers enhance the migration of stimulated Langerhans cells to draining lymph nodes and induce effective transcutaneous immunization.
- Source :
- Nanomedicine: Nanotechnology, Biology & Medicine; Jan2014, Vol. 10 Issue 1, p215-223, 9p
- Publication Year :
- 2014
-
Abstract
- Abstract: This study aims to investigate the efficacy of chitosan nanoparticles (CS-NPs) as a vehicle for transcutaneous antigen delivery in anti-tumor therapy. Ovalbumin (OVA) or gp100 (melanocyte-associated antigen gp100 protein)-loaded CS-sodium tripolyphosphate (TPP)-grafted NPs were prepared by crosslinking low-molecular-weight CS with TPP. Compared with the FITC-OVA solution, the encapsulated fluorescein isothiocyanate (FITC)-OVA-loaded NPs expressed much stronger cellular uptake ability in vitro and higher ability to migrate to lymph nodes in vivo. After transcutaneous administration, OVA-loaded NPs, with imiquimod as an adjuvant, increased the anti-OVA immunoglobulin G titer to levels similar to those induced by the OVA solution. The gp100-loaded NPs promoted the survival of tumor-bearing mice. These results provided evidence of CS-NPs as promising carriers for transcutaneous vaccine delivery, partly contributing to the increased uptake of NPs by skin antigen-presenting cells as well as their enhanced migration to the surrounding lymph nodes. From the Clinical Editor: In this study the efficacy of chitosan nanoparticle based vehicles for transcutaneous antigen delivery is investigated in anti-tumor therapy. Authors demonstrate that such nanoparticles may be efficient carriers partly due to their increased uptake by antigen-presenting cells in the skin and their enhanced migration to surrounding lymph nodes. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 15499634
- Volume :
- 10
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Nanomedicine: Nanotechnology, Biology & Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 93334652
- Full Text :
- https://doi.org/10.1016/j.nano.2013.06.007