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An Infection-Relevant Transcriptomic Compendium for Salmonella enterica Serovar Typhimurium.
- Source :
- Cell Host & Microbe; Dec2013, Vol. 14 Issue 6, p683-695, 13p
- Publication Year :
- 2013
-
Abstract
- Summary: Bacterial transcriptional networks consist of hundreds of transcription factors and thousands of promoters. However, the true complexity of transcription in a bacterial pathogen and the effect of the environments encountered during infection remain to be established. We present a simplified approach for global promoter identification in bacteria using RNA-seq-based transcriptomic analyses of 22 distinct infection-relevant environmental conditions. Individual RNA samples were combined to identify most of the 3,838 Salmonella enterica serovar Typhimurium promoters in just two RNA-seq runs. Individual in vitro conditions stimulated characteristic transcriptional signatures, and the suite of 22 conditions induced transcription of 86% of all S. Typhimurium genes. We highlight the environmental conditions that induce the Salmonella pathogenicity islands and present a small RNA expression landscape of 280 sRNAs. This publicly available compendium of environmentally controlled expression of every transcriptional feature of S. Typhimurium constitutes a useful resource for the bacterial research community. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 19313128
- Volume :
- 14
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- Cell Host & Microbe
- Publication Type :
- Academic Journal
- Accession number :
- 92901366
- Full Text :
- https://doi.org/10.1016/j.chom.2013.11.010