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Mechanism of Inhibition of Tubuloglomerular Feedback by CO and cGMP.
- Source :
- Hypertension (0194911X); Jul2013, Vol. 62 Issue 1, p99-104, 6p
- Publication Year :
- 2013
-
Abstract
- Tubuloglomerular feedback (TGF) is a mechanism that senses NaCl in the macula densa (MD) and causes constriction of the afferent arteriole. CO, either endogenous or exogenous, inhibits TGF at least in part via cGMP. We hypothesize that CO in the MD, acting via both cGMP-dependent and -independent mechanisms, attenuates TGF by acting downstream from depolarization and calcium entry into the MD cells. In vitro, microdissected rabbit afferent arterioles and their MD were simultaneously perfused and TGF was measured as the decrease in afferent arteriole diameter. MD depolarization was induced with ionophores, while adding the CO-releasing molecule-3 to the MD perfusate at nontoxic concentrations. CO-releasing molecule-3 blunted depolarization-induced TGF at 50 µmol/L, from 3.6±0.4 to 2.5±0.4 µm (P<0.01), and abolished it at 100 µmol/L, to 0.1±0.1 urn (P<0.001; n=6). When cGMP generation was blocked by guanylyl cyclase inhibitor LY83583 added to the MD, CO-releasing molecule-3 no longer affected depolarization-induced TGF at 50 µmol/L (2.9±0.4 versus 3.0±0.4 µm) but partially inhibited TGF at 100 µmol/L (to 1.3±0.2 urn; P<0.05; n=9). Experiments using eicosatetraynoic acid and indomethacin suggest arachidonic acid metabolites do not mediate the cGMP-independent effect of CO. We then added the calcium ionophore A23187 to the MD, which caused TGF (4.1+0.6 µmol/L); A23187-induced TGF was inhibited by CO-releasing molecule-3 at 50 µmol/L (1.9+0.6 µmol/L; P<0.01) and 100 µmol/L (0.2±0.5 µmol/L; P<0.001; n=6). We conclude that CO inhibits TGF acting downstream from depolarization and calcium entry, acting via cGMP at low concentrations, but additional mechanisms of action may be involved at higher concentrations. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0194911X
- Volume :
- 62
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Hypertension (0194911X)
- Publication Type :
- Academic Journal
- Accession number :
- 88788798
- Full Text :
- https://doi.org/10.1161/HYPERTENSIONAHA.113.01164