Paclitaxel loaded poly-d,l-lactide-co-glycolide nanoparticles: Enhanced anticancer effect in non-small cell lung carcinoma cell line.

Abstract: The aim of the present study was to develop PTX-PLGA nanoparticles and to study its anticancer efficacy in NCI-H460 cells in vitro. DLS results revealed that prepared PTX-PLGA had an average size of 211nm. TEM analysis showed that prepared PTX-PLGA nanoparticles were spherical in shape with 150nm. Drug encapsulation assay showed that 65% PTX was encapsulated in PLGA. It was noticed that anticancer potential of PTX-PLGA nanoparticles was greater than that of PTX treatment alone. Further, PLGA-PTX exhibits increased intracellular ROS levels, altered MMP, increased DNA damage, enhanced TBARS and apoptotic morphological changes in NCI-H460 cells when compared to bulk PTX treatment alone. Our results indicate that PTX-PLGA demonstrated increased anticancer property in cancer cells than PTX treatment alone. [Copyright &y& Elsevier]