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Cloning and sequence analysis of Toll-like receptor 9 gene from sika deer.

Authors :
Li-chun, ZHANG
Quan-kai, WANG
CAO Yang
ZHAO Xue
Zhao-zhi, LI
Xiao-yang, WANG
Qing-lin, PIAO
Hai-guo, JIN
Source :
Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao; 2012, Vol. 32 Issue 9, p1386-1391, 6p
Publication Year :
2012

Abstract

The article studies cloning and sequence analysis of Toll-like receptor 9 gene from sika deer. It informs that total RNA from liver tissue was extracted for sequencing and the Toll-like receptor 9 gene (TLR9) was cloned by real-time polymerase chain reaction (RT-PCR) with degenerate primers which was designed to locate on two ends of open reading frames (ORF). It informs that deer TLR9 gene was analyzed by bio-informatics software.

Details

Language :
English
ISSN :
10054545
Volume :
32
Issue :
9
Database :
Supplemental Index
Journal :
Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao
Publication Type :
Academic Journal
Accession number :
84529001