Comparative Evaluation between Two Acoustic Immunosensors: Love-wave and QCM, and Systems of Measurement: Dynamic and Static.

Abstract: The goal has been to develop a Love-wave device and a PDMS microfluidic chip in order to measure immunoreactions in liquid media, operating in dynamic mode (continuous flow-through). The obtained results have been compared with the most used acoustic wave device, the quartz crystal microbalance (QCM), which worked in static mode. It has been demonstrated once again that QCM is an excellent tool as immunosensor, although Lovewave device is a good alternative due to its high sensitivity. Besides, the response time and limit of detection are improved working in dynamic mode. On the other hand, a secondary antibody conjugated with gold nanoparticles has been used as a method to measure concentrations of antigens obtaining a great frequency shift. [Copyright &y& Elsevier]