Screening, identification, and phosphate-solubilizing capability of phosphate-solubilizing bacterial strain B25.

Various phosphatesolubilizing bacterial strains were isolated from the Hippochaete ramo sissimum rhizosphere in Tongguanshan copper tailings in Tongling of Anhui Province, East China. After many times of screening and purification, a strain B25 with stronger phosphatesolubilizing ca pability was obtained, which belonged to Bacillus genus, as identified by transmission electron microscope and DNA molecular approaches. A culture experiment was conducted to study the phosphate-solubilizing capability of the B25 within 168 h and the variations of the medium pH and B25 growth as well as the phosphate-solubilizing capability of B25 under different culture conditions. A weak correlation was observed between the phosphate-solubilizing capability of B25 and the medium pH. The B25 displayed a better phosphate-solubilizing capability when the carbon source was glucose, medium initial pH was 7. 0, and culture temperature was 30 °C. [ABSTRACT FROM AUTHOR]