Effect of HPV 16 E5 on E6 and E7 gene induced human immortalized oral epithelial cell.

PURPOSE: To investigate the influence of HPVI6 E5 on E6 and E7 gene in human oral immortalized epithelial cells (HIOEC). METHODS: The pLEGFP-E5 was transferred into HIOEC cell; Deletion mutation expression vectors were constructed and transferred into HIOEC; RT-PCR was used to detect the gene expression in HIOEC; The expression of E6 anti E7 mRNA was detected by real time-PCR;Cell proliferation of HIOEC was evaluated by MIT. All statistical analysis was performed by using SPSS 13.0 software package. RESULTS: The deletion mutation expression vector of E5 was successfully constructed and applied for further experiments; E5 and the deletion mutation genes were transferred into HIOEC successfully; HPVI6 E5 promoted cell proliferation of HIOEC and made the increased expression raised of E6 and E7 genes; However, the deletion mutation had no significant effect on HIOEC. CONCLUSIONS: HPVI6 E5 is transferred into HIOEC successfully. E5 may promote cell proliferation and upregulate the mRNA expression of E6 and E7 genes. Supported by Natural Science Foundation of Anhui Province (070413086). [ABSTRACT FROM AUTHOR]