Development of a real-time polymerase chain reaction assay for detection of Actinobacillus subs in porcine lung.

The article discusses a study, which describes the development and validation of a real-time polymerase chain reaction (PCR) assay using a TaqMan-labeled probe for the detection of Actinobacillus suis in porcine lung. Actinobacillus suis is Gram-negative bacterium that is considered an important swine pathogen because of its ability to cause fatal disease in pigs. The study found that the TaqMan real-time assay is a highly specific, sensitive, and reproducible test.