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Effects of applying different nitrogen form on cherry tomato nitrogen metabolism during fruit development.
- Source :
- Chinese Journal of Applied Ecology / Yingyong Shengtai Xuebao; Sep2010, Vol. 21 Issue 9, p2335-2341, 7p, 1 Black and White Photograph, 3 Charts, 3 Graphs
- Publication Year :
- 2010
-
Abstract
- A hydroponic experiment was conducted to study the effects of applying 100% NO<subscript>3</subscript><superscript>-</superscript>-N,100% NH<subscript>4</subscript><superscript>+</superscript>-N, and 75% NO<subscript>3</subscript><superscript>-</superscript>-N+25% NH<subscript>4</subscript><superscript>+</superscript>-N on the nitrogen metabolism and the nitrate reductase (NR) and glutamine synthetase (GS) gene expression of cherry tomato during its fruit development. Applying 75% NO<subscript>3</subscript><superscript>-</superscript>-N+25% NH<subscript>4</subscript><superscript>+</superscript>-N slightly increased the single fruit mass, and increased the fruit NH<subscript>4</subscript><superscript>+</superscript>-N, total amino acid, and total N contents and N accumulation significantly, compared with applying 100% NO<subscript>3</subscript><superscript>-</superscript>-N. In treatments 100% NO<subscript>3</subscript><superscript>-</superscript>-N and 75% NO<subscript>3</subscript><superscript>-</superscript>-N+25% NH<subscript>4</subscript><superscript>+</superscript>-N, the fruit NR activity and its gene expression had no significant difference, but were higher than those in treatment 100% NH<subscript>4</subscript><superscript>+</superscript>-N. The fruit GS activity was significantly higher in treatment 75% NO<subscript>3</subscript><superscript>-</superscript>-N+25% NH<subscript>4</subscript><superscript>+</superscript>-N than in treatment 100% NO<subscript>3</subscript><superscript>-</superscript>-N. In the three treatments, isozyme GS1 (Cytosolic type GS) and GS2 (Chloroplast type GS) expression was inconsistent with GS activity, suggesting that the effects of applied N on GS activity could be mainly reflected at posttranscriptional level. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Chinese
- ISSN :
- 10019332
- Volume :
- 21
- Issue :
- 9
- Database :
- Supplemental Index
- Journal :
- Chinese Journal of Applied Ecology / Yingyong Shengtai Xuebao
- Publication Type :
- Academic Journal
- Accession number :
- 63536078