Development and validation of a TaqMan® PCR assay for the Australian abalone herpes-like virus.

Authors :: Corbeil, Serge
Coiling, Axel
Williams, Lynette M.
Wong, Frank Y. K.
Savin, Keith
Warner, Simone
Murdoch, Bronwyn
Cogan, Noel O. I.
Sawbridge, Timothy I.
Fegan, Mark
Mohammad, Ilhan
Sunarto, Agus
Handlinger, Judith
Pyecroft, Stephen
Douglas, Marianne
Chang, Pen H.
Crane, Mark St. J.
Source :: Diseases of Aquatic Organisms; 10/26/2010, Vol. 92 Issue 1, p1-10, 10p, 5 Charts, 6 Graphs, 1 Map
Publication Year :: 2010
Abstract: This article discusses a study on the development, optimization and validation of a real-time TaqMan assay for abalone herpes-like virus (AbHV). The study used AbHV-infected wild blacklip abalone Haliotis rubra from coastal waters in Victoria. The assay detected DNA from the Taiwanese abalone herpes-like virus. The detection suggests a relationship between the Taiwanese and Australian viruses. The assay also detected copies of recombinant plasmid DNA per reaction. The study also revealed the highest diagnostic sensitivity and specificity.

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