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Possible mechanism of betel-quid-extract-induced expression of matrix metalloproteinase-2.
- Source :
- Journal of the Formosan Medical Association; Nov2010, Vol. 109 Issue 11, p838-847, 10p
- Publication Year :
- 2010
-
Abstract
- <bold>Background/purpose: </bold>Betel quid extract (BQE) has been demonstrated to induce matrix metalloproteinase (MMP)-2 expression. This study aimed to establish the possible mechanism involved in this event.<bold>Methods: </bold>Western blotting, reverse-transcription polymerase chain reaction, and gelatin zymography were used to study the expression level of MMP-2. LY294002, PD98059, U0126, N-acetyl-L-cysteine, SB203580, SP600125, and Bay 11-7082 were used to pretreat OECM-1 cells before BQE treatment and MMP-2 detection.<bold>Results: </bold>OECM-1 cells were subjected to short-term (10 minutes) or long-term (24 hours) BQE treatment (designated as SBT and LBT, respectively), and we found that both treatments increased MMP-2 protein and extracellular signal-regulated kinase (ERK) phosphorylation levels in a concentration- and time-dependent manner. LBT also increased MMP-2 mRNA level. LBT-induced MMP-2 secretion was not inhibited by PD98059 (up to 50 μM) when ERK was effectively blocked, but was attenuated by LY294002 (0-10 μM) in a concentration-dependent manner. This LBT effect was inhibited strongly by SB203580 (10 μM), SP600125 (10 μM), and Bay 11-7082 (10 μM) and mildly by N-acetyl-L-cysteine (5 mM), but not by U0126 (10 μM).<bold>Conclusion: </bold>Both SBT and LBT upregulate MMP-2 expression, and LBT-induced MMP-2 expression might be mediated by phosphoinositide 3-kinase, p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and nuclear factor-κB, and to a lesser extent, by reactive oxygen species, rather than by ERK. [ABSTRACT FROM AUTHOR]
- Subjects :
- METALLOPROTEINASES
POLYMERASE chain reaction
GELATIN
CYSTEINE proteinases
PHOSPHORYLATION
PHOSPHOINOSITIDES
PROTEIN kinases
MESSENGER RNA
BETEL leaves
ANIMAL experimentation
BETEL palm
BIOCHEMISTRY
COMPARATIVE studies
DOSE-effect relationship in pharmacology
MASTICATION
PHENOMENOLOGY
RESEARCH methodology
MEDICAL cooperation
MICE
MOUTH tumors
PROTEOLYTIC enzymes
RESEARCH
RNA
SQUAMOUS cell carcinoma
TIME
WESTERN immunoblotting
EVALUATION research
PLANT anatomy
REVERSE transcriptase polymerase chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 09296646
- Volume :
- 109
- Issue :
- 11
- Database :
- Supplemental Index
- Journal :
- Journal of the Formosan Medical Association
- Publication Type :
- Academic Journal
- Accession number :
- 55504365
- Full Text :
- https://doi.org/10.1016/S0929-6646(10)60129-5