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Simple Purification of a Foreign Protein Using Polyhedrin Fusion in a Baculovirus Expression System.

Authors :
Jong Yul Roh
Jae Young Choi
Joong Nam Kang
Yong Wang
Hee Jin Shim
Qin Liu
Xueying Tao
Hong Guang Xu
Jin-Ho Hyun
Soo Dong Woo
Byung Rae Jin
Yeon Ho Je
Source :
Bioscience, Biotechnology & Biochemistry; Aug2010, Vol. 74 Issue 8, p1522-1526, 5p, 1 Chart, 1 Graph
Publication Year :
2010

Abstract

The article presents a study concerning the purification of a foreign protein. The study shows that the fusion of polyhedrin (Polh)-green fluorescene protein (GFP) will lead to the formation of granular structures. It shows that Polh fusion and enterokinase digestion can be used for easy and fast purification of other proteins.

Details

Language :
English
ISSN :
09168451
Volume :
74
Issue :
8
Database :
Supplemental Index
Journal :
Bioscience, Biotechnology & Biochemistry
Publication Type :
Academic Journal
Accession number :
55154317
Full Text :
https://doi.org/10.1271/bbb.100016