SPECTROSCOPIC STUDY OF HUMIC SUBSTANCES ISOLATED FROM SEDIMENT AND WATER.

NMR, EPR and JR spectroscopic studies of humic substances isolated from sediment and water samples have been used for characterization of humification process occurring in the Goczalkowice Reservoir. Fulvic acids (FA) were isolated from both sediment and water samples whereas humic acids (HA) only from the former due to their absence in the latter. Humic substances were studied with JR spectroscopy in order to suggest the presence of functional chemical groups. Comparison of signal intensity of the main functional groups was conducted by means of tHNIvIR techniques. The concentration of free radicals was determined with EPR. It was found that free radical concentrations of FA isolated from sediment are considerably higher than those of FA from water and HA from sediment. On the basis of the interpretation of EPR and JR spectra, the formation of transition metal complexes with humic substances was confirmed. [ABSTRACT FROM AUTHOR]