Cross-Specific Multiplexed Microsatellite Set for Brazilian Buffalo Identification.

Buffaloes (Bubalus bubalis) were introduced to Brazil in 1895. Many authors have used microsatellites for genetic characterization of buffalo, but only some papers include molecular markers in Brazilian populations. Cross-species utility of microsatellites is one of the preferred marker choice criteria. To develop a multiplexed set of microsatellites and characterize Brazilian buffalo 19 cattle microsatellites were tested. Three panels were finally developed with eleven that succeed in amplify and were polymorphic (58%). The average number of alleles was 5.42, with an average observed and expected heterocigozity of 0.441 and 0.695, respectively. Calculated overall exclusion power considering on e parent, two parents available and sample matching were 0.98, 0.9991 and 0.9999999996, respectively. The set has an exclusion power in Brazilian buffalo that allows its use in DNA based traceability and some paternity cases, such as multi-sire breeding scenarios, when one parent is known and could be genotyped. Furthermore, highly informative tested microsatellites could be added to existing panels. [ABSTRACT FROM AUTHOR]