Optimization of intracellular polymer analysis for enhanced biological phosphorous removal system.

The combination of chromatogram and chemical analysis was adopted to optimize the methods selected to measure poly - P, glycogen and PHA intracellular polymer in EBPR system. PHA was measured by GC system, glycogen was degraded by dilute hydrochloric acid and analyzed by glucose commercial kit, and poly - P was oxidized by potassium persulfate and analyzed by molybdenum - antimony anti-spectrophotometric method. Testing of the proposed method for PHA analysis shows that 4% sulfuric acid concentration and degradation for 20 h are required for optimal PHB and PHV extraction, while 0.6 mol/L HCl and digestion for 5 h produce the highest recovery of glycogen, and the medical glucose kit can be used in this research. Oxidation for 30 min at 120 °C is recommended for the recovery of poly - P. The results of precision analysis and recovery test with standard addition of these methods show that they possess high precision and are convenient for laboratory research and practical application. [ABSTRACT FROM AUTHOR]