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Expression of porcine interferon-alpha using a baculovirus and its inhibition activity on the replication of PRRSV.

Authors :
Wang Yari-Bin
Huang Yan-qun
Cut Bao-an
Chen Hong-ying
Wang Ya-bin
Zhang Hong-ying
Source :
Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao; 2009, Vol. 29 Issue 12, p1522-1526, 5p, 6 Diagrams, 1 Chart
Publication Year :
2009

Abstract

The article presents a study regarding the gene expression of a mature porcine interferon-alpha (PoIFN-α) fused with C-terminal 6 x Histidine tag using the baculovirus pFastBac™ I vector. The recombinant proteins of the gene expression were detected by Western blotting analysis and immunofluorescence assays. The results of the study indicate the therapeutic effect of PoIFN-α which inhibits the replication of porcine reproductive and respiratory syndrome virus (PRRSV).

Details

Language :
Chinese
ISSN :
10054545
Volume :
29
Issue :
12
Database :
Supplemental Index
Journal :
Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao
Publication Type :
Academic Journal
Accession number :
51822228