Experimental study on demulsification characteristics of water-in-oil emulsions by a Bacillus mojavensis sp.

To accurately regulate the production of active substances with demulsification characteristic by biological demulsification bacteria, the effects of different culture time, different components of culture solution and different types of carbon source and nitrogen source on a Bacillus mojavensis sp., which was isolated by our laboratory, were determined by demulsifying the O/W model emulsions, respectively. Results show that when the culture time is 14 -- 24 h, the highest demulsification activity of culture solution can be achieved. The culture and cell -- free culture have the high efficiency of demulsification while the bacterial cells do not. Hydrocarbons carbon source can induce this strain to produce the metabolites with demulsification activity and carbohydrate carbon source can promote the growth of cells. The mix carbon source containing fructose and liquid paraffin is most favorable for the growth and demulsification. Compared with the single nitrogen source, the mix nitrogen source containing ammonium nitrate and yeast extract is more beneficial to the growth and the demulsification production of this strain. The level of demulsifying activity of metabolites from this strain can be controlled by regulating the culture time, carbon source and nitrogen source. [ABSTRACT FROM AUTHOR]