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Cytotoxicity and Degree of Conversion of Orthodontic Adhesives.
- Source :
- Angle Orthodontist; Nov2009, Vol. 79 Issue 6, p1133-1138, 6p
- Publication Year :
- 2009
-
Abstract
- Objectives: To test the hypothesis that there is no difference in the cytotoxicity related to the modes of polymerization of five commercially available orthodontic bonding resins, with and without an oxygen-inhibited layer (OIL), and to evaluate the degree of conversion (DC) of these resins and correlate this to cytotoxicity. Materials and Methods: Five commercially available orthodontic bonding resins were tested for cytotoxicity and DC. Thirty-six disks of standardized dimensions, for each resin, were used for cytotoxicity assessment. Half of them were washed with 99% acetone to remove the OIL (washed resins), and the remaining disks were left intact (intact resins). Glass disks were used as a control. Vero cells were exposed to intact and washed resins on day 1. Cell viability was determined by tetrazolium bromide reduction assay 1, 3, and 6 days after exposure. The DC of the adhesive specimens of each resin, prepared with a procedure identical to the clinical bonding process, was assessed by Fourier transform infrared spectroscopy. Results: Single-cured systems were comparatively less cytotoxic than dual-cured systems. With removal of the OIL, increased cell viability was noted only with two resins on all three days. Resins tested showed differences in DC. A positive correlation was demonstrated by two resins. Conclusion: The hypothesis is rejected. Single-cured systems are superior to dual-cured systems in exhibiting comparatively less toxicity and higher DC. A significant positive correlation was not established between cytotoxicity and DC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00033219
- Volume :
- 79
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- Angle Orthodontist
- Publication Type :
- Academic Journal
- Accession number :
- 45689424
- Full Text :
- https://doi.org/10.2319/080808-418R.1